INDUCTION BY 5-FLUOROURACIL OF BACTERIOPHAGE DEVELOPMENT IN LYSOGENIC E. COLI K12 (/lambda/)

Journal Article · Thu Oct 31 23:00:00 EST 1963 · Nature

DOI:https://doi.org/10.1038/200487a0· OSTI ID:4152254

Marcovich, H; Kaplan, H S

E. coli K12 ( lambda ), strain B-40, streptornycin-sensitive was grown to about 5 x 10/sup 9/ cells/ml at 37 deg C in modified Davis mineral riedium to which 0.8% glucose and 5 mg/ml vitamin-free casein hydrolysate were added. Preliminary experiments in which E. coli K12 ( lambda ) were incubated for at least 60 min in the presence of high concentrations (50-100 mu g/ml) of 5- fluorouracil (FU) or its deoxyriboside (FUDR) revealed that a burst of phage production occurred after the subsequent addition of thymine (T) or thymidine (TdR) plus uracil (U) or uridine (UR). In the presence of FU alone, an initial decrease in phage titre was found, followed within 30--60 min after the addition of TdR by a sharp rise that attained levels about 1000-fold higher in approximately 1 hr. In another experiment the addition of TdR to FU containing cultures at 90 min was followed within 1 hr by a sharp burst of phage production; the response was completely preverted when the same concentration of TdR was added at 0 min. Thus it was found that the initial step in phage induction by FU requires a period of time for the action of the analogue in the absence of T or TdR. (P.C.H.)

• Research Organization: Institut Pasteur, Paris
• Sponsoring Organization: USDOE
• NSA Number: NSA-18-001336
• OSTI ID: 4152254
• Journal Information: Nature, Journal Name: Nature Vol. Vol: 200
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANTIBIOTICS
BACTERIA
BACTERIOPHAGES
BIOLOGY AND MEDICINE
CASEIN
CYTOLOGY
ENVIRONMENT
ESCHERICHIA COLI
GLUCOSE
HETEROCYCLICS
KETONES
MINERALS
NUCLEIC ACIDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRODUCTION
PROTEINS
PYRIMIDINES
QUANTITY RATIO
SENSITIVITY
STREPTOMYCIN
SUGARS
TEMPERATURE
THYMIDINE
URACILS
URIDINE
VARIATIONS