Mechanism of nephrotoxicity induced by repeated administration of cadmium chloride in rats

Journal Article · Mon Jul 01 04:00:00 EDT 1996 · Journal of Toxicology and Environmental Health

DOI:https://doi.org/10.1080/009841096161230· OSTI ID:415115

Sudo, Jun-ichi; Hayashi, Taiji; Kimura, Shin-ichi ^[1]

---

1. Health Sciences Univ., Ishikari-Tobetsu, Hokkaido (Japan); and others

To explore the mechanism of Cd nephrotoxicity, CdCl{sub 2} was subcutaneously injected to rats, at 3 mg Cd/kg body weight once a day, for 8 d. In the liver, Cd bound to metallothioneins (MTs-Cd) rose from d 1 after the initiation of CdCl{sub 2} administration, and reached a plateau after the administration ceased. In the plasma, MTs-Cd rose from d 4, peaked on d 8, and gradually fell thereafter. In the kidney`s leucine aminopeptidase (LAP) and N-acetyl {beta}-D-glucosaminidase (NAG) fell during d 6-20, and Cd bound to cellular membranes (Mem-Cd) rose from d 1 and reached a plateau during d 6-20. The Mem-Cd levels were significantly correlated with the reduction in the LAP and NAG activity; the values of MTs-Cd plus Mem-Cd were almost equivalent to those of total Cd. These findings showed that the hepatic synthesis of MTs-Cd occurred followed by its release into plasma; the extent of renal injury was aggravated as the plasma level of MTs-Cd rose; and a greater part of the renal Cd distributed intracellularly as the MTs-binding form, while the residual Cd distributed as the cellular membrane-binding form. Also, it was suggested that Cd that occurred as the cullular membrane-binding form in the kidneys was involved in manifestation of renal injury. 29 refs., 9 figs., 1 tab.

• Sponsoring Organization: USDOE
• OSTI ID: 415115
• Journal Information: Journal of Toxicology and Environmental Health, Journal Name: Journal of Toxicology and Environmental Health Journal Issue: 4 Vol. 48; ISSN 0098-4108; ISSN JTEHD6
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL EFFECTS
CADMIUM
KIDNEYS
RATS
UROGENITAL SYSTEM DISEASES