THE CYTOLOGY AND GENETICS OF RADIATION RESISTANCE IN BACTERIA. Progress Report for the Period of November 1, 1955 to November 1, 1956
Cytogenetic procedures, applicable to microbiology, were selected and tested on a suitable organism as a basis for the valid application of these procedures to other microorganisms. Nocardia corallina was chosen as a test organism on the basis of preliminary cytological studies. The crystal violet nuclear stain, the thionin-SO/sub 2/ nuclear stain, the crystal violet-tannic acid-congo red cell wall stain, and phase microscopy, were found to be valid tools of microbial cytology if interpreted with restraint. The correlation of cytological and radiobiological findings demonstrated that, in N. corallina, a diploid coccoidal stage, gives rise to a coenocytic diploid hypbal stage which fragments through a nuclear reduction division to form haploid dinucleated bacillary cells. The bacillary cell nuclei fuse and the cell divides to form diploid coccoids. The haploid chromosome number is suggested as three for this organism. It has been demonstrated that a microbial cytogenetic approach involving the correlation and integration of cytological procedures with genetic and radiobiological methods can aid in solving basic problems of microbial cytology and genetics. (For preceding period see ORO-282.) (auth)
- Research Organization:
- Oklahoma. Univ., Norman. Research Inst.
- DOE Contract Number:
- AT(40-1)-1976
- NSA Number:
- NSA-14-017693
- OSTI ID:
- 4150417
- Report Number(s):
- ORO-283
- Resource Relation:
- Other Information: Orig. Receipt Date: 31-DEC-60
- Country of Publication:
- United States
- Language:
- English
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THE CYTOLOGY AND GENETICS OF RADIATION RESISTANCE IN BACTERIA. Progress Report for the Period of November 1, 1956 to November 1, 1957
THE CYTOLOGY AND GENETICS OF RADIATION RESISTANCE IN BACTERIA. Progress Report for the Period of November 1, 1957 to November 1, 1958