HOMOGRAFT-REACTIVE LARGE MONONUCLEAR LEUKOCYTES IN PERIPHERAL BLOOD AND PERITONEAL EXUDATES
Technical Report
·
OSTI ID:4148418
Two criteria have been employed for demonstrating the presence of homograft-reactive cells in the peripheral blood and in peritoneal exudates: the production of a lethal wasting syndrome in sublethally x-irradiated (500 r) rental strain (A strain) donor mice and the abrogation of protection by injected bone marrow in lethally x-irradiated mice by these cells. The injection of 8 x 10/sup 6/ peritoneal exudate granulocytes does not elicit the wasting syndrome; whereas 2.1 x 10/sup 6/ mononuclear cells obtained from peritoneal exudates 8 days after mineral-oil administration are reactive. When peritoneal mononuclear cells from isoimmunized A-strain donors were administered, reactivity of the cells was enhanced. Prior treatment of A-strain donor mice with cortisone did not abolish the homograft reactivity of the injected blood (1.1 x 10/sup 6/ nucleated cells). Under these conditions of cortisone treatment, there was a relative increase in the frequency of large mononuclear cells (9 mu diameter or larger) and a disappearance of essentially all the small lymphocytes (5 mu --7 mu diameter). Data are presented which indicate the capacity of the large mononuclear cells in peripheral blood to transform into cells of the plasma cell series. It is concluded that the circulating blood contains a mobile pool of large mononuclear cells capable of division, and potentially able to initiate immunological reactions (i.e., homograft reactions) analogous to that possessed by lymphoid tissue cells. Although the present experiments deal with specific inbred mouse strains, their possible implications for bone marrow therapy in irradiated humans are evident. (auth)
- Research Organization:
- Naval Radiological Defense Lab., San Francisco
- NSA Number:
- NSA-14-025152
- OSTI ID:
- 4148418
- Report Number(s):
- USNRDL-TR-446
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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