Photodynamic inactivation of antigenic determinants of single-stranded DNA bacteriophage phiX174
Bacteriophage phi X174 when photodynamically inactivated (i.e., when rendered unable to produce plaques as a result of exposure to visible light in air in the presence of proflavine) progressively lost their capacity to bind efficiently with homologous antiserum. Such loss of serum-blocking power was evident with heat-inactivated but not with uv-irradiated phage. The ability of the phages to adsorb to host cells, however, remained practically unaltered even after photodynamic inactivation. It thus appears that photodynamic damages in the so-called ''jacket'' component of the phi X174 coat proteins are partly responsible for the loss of plaque-forming ability, whereas the ''spikes'' are either poor antigens or insensitive to photodynamic treatment. (auth)
- Research Organization:
- Saha Inst. of Nuclear Physics, Calcutta
- NSA Number:
- NSA-33-000881
- OSTI ID:
- 4143413
- Journal Information:
- J. Virol., v. 13, no. 5, pp. 997-1000, Other Information: Orig. Receipt Date: 30-JUN-76
- Country of Publication:
- United States
- Language:
- English
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