Role for deoxyribonucleic acid ligase in deoxyribonucleic acid polymerase I- dependent repair synthesis in toluene-treated Escherichia coli

Journal Article · Wed Oct 01 04:00:00 EDT 1975 · J. Bacteriol., v. 124, no. 1, pp. 585-588

OSTI ID:4137090

Billen, D; Hellermann, G R; Stallions, D R

In a toluene-treated mutant of Escherichia coli K-12 having a temperature-sensitive, conditionally lethal mutation in the structural gene for deoxyribonucleic acid (DNA) ligase, an extensive DNA repair synthesis occurred in x-irradiated cells at the nonpermissive temperature, 42$sup 0$C. At the permissive temperature, 30$sup 0$C, nearly normal semiconservative synthesis and limited repair synthesis were observed when DNA ligase was activated by the addition of nicotinamide adenine dinucleotide. (auth)

• Research Organization: Oak Ridge National Lab., TN
• NSA Number: NSA-33-003172
• OSTI ID: 4137090
• Journal Information: J. Bacteriol., v. 124, no. 1, pp. 585-588, Journal Name: J. Bacteriol., v. 124, no. 1, pp. 585-588; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

*DNA-- BIOLOGICAL REPAIR
*ESCHERICHIA COLI-- BIOLOGICAL RADIATION EFFECTS
*LIGASES-- METABOLISM
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
BIOSYNTHESIS
CATALYSIS
GENES
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
NUCLEOTIDES
POLYMERASES
TEMPERATURE DEPENDENCE
TOLUENE
X RADIATION