Effect of Glycerine on the X-Ray Sensitivity of Serratia Marcescens
Radiation protection by glycerine was examined using the smaIl red- pignnented bacterium Serratia marcescens. Observations indicated that the protection of aerobic organisms was brought about by some mechanism other than oxygen scavenging or competition with oxygen for radioinduced radicals. Organisms that were anaerobic when irradiated were also substantially protected by the presence of glycerine. Overnight cultures grown in unaerated nutrient were diluted in phosphate buffer and suspended in glycerine solution a few minutes before irradiation. The protective effect of glycerine was found to be independent of a change in oxygen concentration by a factor of 100 and also independent of temperature changes. The ratio of sensitivity of the bacteria in the absence of glycerine to its sensitivity in glycerine was found to be 5.25.
- Research Organization:
- Mount Vernon Hospital, Northwood, Middx., Eng.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-14-025337
- OSTI ID:
- 4136630
- Journal Information:
- Nature (London), Journal Name: Nature (London) Journal Issue: 4742 Vol. 187; ISSN 0028-0836
- Publisher:
- Nature Publishing Group
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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