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Title: Effect of Glycerine on the X-Ray Sensitivity of Serratia Marcescens

Abstract

Radiation protection by glycerine was examined using the smaIl red- pignnented bacterium Serratia marcescens. Observations indicated that the protection of aerobic organisms was brought about by some mechanism other than oxygen scavenging or competition with oxygen for radioinduced radicals. Organisms that were anaerobic when irradiated were also substantially protected by the presence of glycerine. Overnight cultures grown in unaerated nutrient were diluted in phosphate buffer and suspended in glycerine solution a few minutes before irradiation. The protective effect of glycerine was found to be independent of a change in oxygen concentration by a factor of 100 and also independent of temperature changes. The ratio of sensitivity of the bacteria in the absence of glycerine to its sensitivity in glycerine was found to be 5.25.

Authors:
Publication Date:
Research Org.:
Mount Vernon Hospital, Northwood, Middx., Eng.
Sponsoring Org.:
USDOE
OSTI Identifier:
4136630
NSA Number:
NSA-14-025337
Resource Type:
Journal Article
Journal Name:
Nature (London)
Additional Journal Information:
Journal Volume: 187; Journal Issue: 4742; Other Information: Orig. Receipt Date: 31-DEC-60; Journal ID: ISSN 0028-0836
Publisher:
Nature Publishing Group
Country of Publication:
Country unknown/Code not available
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ALCOHOLS; BACTERIA; FREE RADICALS; GLYCERIN; OXYGEN; RADIATION PROTECTION; RADIOSENSITIVITY; SERRATIA; VARIATIONS

Citation Formats

DEWEY, D. L. Effect of Glycerine on the X-Ray Sensitivity of Serratia Marcescens. Country unknown/Code not available: N. p., 1960. Web. doi:10.1038/1871008a0.
DEWEY, D. L. Effect of Glycerine on the X-Ray Sensitivity of Serratia Marcescens. Country unknown/Code not available. doi:10.1038/1871008a0.
DEWEY, D. L. Sat . "Effect of Glycerine on the X-Ray Sensitivity of Serratia Marcescens". Country unknown/Code not available. doi:10.1038/1871008a0.
@article{osti_4136630,
title = {Effect of Glycerine on the X-Ray Sensitivity of Serratia Marcescens},
author = {DEWEY, D. L.},
abstractNote = {Radiation protection by glycerine was examined using the smaIl red- pignnented bacterium Serratia marcescens. Observations indicated that the protection of aerobic organisms was brought about by some mechanism other than oxygen scavenging or competition with oxygen for radioinduced radicals. Organisms that were anaerobic when irradiated were also substantially protected by the presence of glycerine. Overnight cultures grown in unaerated nutrient were diluted in phosphate buffer and suspended in glycerine solution a few minutes before irradiation. The protective effect of glycerine was found to be independent of a change in oxygen concentration by a factor of 100 and also independent of temperature changes. The ratio of sensitivity of the bacteria in the absence of glycerine to its sensitivity in glycerine was found to be 5.25.},
doi = {10.1038/1871008a0},
journal = {Nature (London)},
issn = {0028-0836},
number = 4742,
volume = 187,
place = {Country unknown/Code not available},
year = {1960},
month = {9}
}