Nuclear labelling after prolonged $sup 3$H-uridine incorporation as visualized by high resolution autoradiography

Journal Article · Fri Jun 01 04:00:00 EDT 1973 · Exp. Cell Res., v. 79, pp. 431-444

DOI:https://doi.org/10.1016/0014-4827(73)90463-1· OSTI ID:4124782

Fakan, S; Bernhard, W

Localization of radioactive labeling over the nuclei of BSC$sub 1$ cells is visualized after long periods of $sup 3$H-5-uridine incubation followed or not followed by periods of postincubation in nonradioactive medium for up to several days, using high resolution autoradiography combined with a preferential staining method for ribonucleoproteins. It is shown that when cells are labeled for 1 or 6 h with $sup 3$H-uridine and postincubated with a nonradioactive medium up to several days, there is always some radioactivity present in the nucleolus and nucleoplasm. When sections of cells fixed after 1 h of labeling followed by 24 h of postincubation are treated with RNase, part of the radioactivity found in the nucleus disappears almost completely only after a succeeding DNase digestion. The majority of interchromatin granules are weakly labeled after most incubation times, with the label localized rather at the periphery of clusters of granules, or are unlabeled. The results are discussed in the context of recent biochemical findings. It is proposed that interchromatin granules might represent a structure containing a limited quantity of slowly labeled nuclear RNA. (auth)

• Research Organization: Inst. Suisse de Recherches Experimentales sur le Cancer, Lausanne, Switzerland
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-009735
• OSTI ID: 4124782
• Journal Information: Exp. Cell Res., v. 79, pp. 431-444, Journal Name: Exp. Cell Res., v. 79, pp. 431-444; ISSN ECREA
• Country of Publication: United States
• Language: English

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*AUTORADIOGRAPHY-- TRITIUM COMPOUNDS
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CELL CONSTITUENTS
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INCUBATION
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URIDINE