Inactivation of adenosine 5'-triphosphate synthesis and reduced-form nicotinamide adenine dinucleotide dehydrogenase activity in Escherichia coli by near-ultraviolet and violet radiations

Journal Article · Thu Jan 01 04:00:00 EST 1976 · J. Bacteriol., v. 125, no. 1, pp. 111-118

OSTI ID:4120691

Lakchaura, B D; Fossum, T; Jagger, J

Near-ultraviolet (near-uv) light (300 to 380 nm) is a significant component of sunlight and has a variety of effects on biological systems. The present work is an attempt to identify chromophores (molecular absorbers of light) and targets (critical damaged molecules) for inhibition of adenosine triphosphate (ATP) synthesis in Escherichia coli by near uv. The fluence at 334 nm required for 37 percent survival of net ATP synthesis (F$sub 37$) in E. coli AB2463 in succinate medium is 140 kJ/m$sup 2$. The action spectrum for this inactivation is almost structureless exhibiting a smooth transition from high efficiency at 313 nm to low efficiency at 405 nm. The action spectrum for inhibition of net ATP synthesis is consistent with the chromophore being either ubiquinone Q-8 or vitamin K$sub 2$. The fluence required is consistent with ubiquinone Q-8 also being a target molecule. The activity of reduced nicotinamide adenine dinucleotide dehydrogenase in extracts of E. coli B is also inactivated by near uv and shows an F$sub 37$ of about 40 kJ/m$sup 2$. The action spectrum for this effect is quite structureless; it shows high efficiency at 313 nm and low efficiency at 435 nm. The data do not suggest a target molecule for this action, although it is possible that ubiquinone Q-8 absorbs the near-uv energy and then passes it on to some other target molecule. The data further indicate that inactivation of the oxidative phosphorylation system is not a primary factor in near-uv-induced growth delay in E. coli. (auth)

• Research Organization: Univ. of Texas, Dallas
• NSA Number: NSA-33-017341
• OSTI ID: 4120691
• Journal Information: J. Bacteriol., v. 125, no. 1, pp. 111-118, Journal Name: J. Bacteriol., v. 125, no. 1, pp. 111-118; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

*ATP-- BIOSYNTHESIS
*DEHYDROGENASES-- METABOLISM
*ESCHERICHIA COLI-- BIOLOGICAL RADIATION EFFECTS
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
CATALYSIS
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
PHOSPHORYLATION
SPECTRA
ULTRAVIOLET RADIATION