Repair replication in permeabilized Escherichia coli

Conference · Wed Jan 01 04:00:00 EST 1975

OSTI ID:4120122

Masker, W E; Simon, T J; Hanawalt, P C; Hanawalt, P C ^[1]

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1. ed.

We have examined the modes of DNA synthesis in Escherichia coli strains made permeable to nucleoside triphosphates by treatment with toluene. In this quasi in vitro system, polymerase-I-deficient mutants exhibit a nonconservative mode of synthesis with properties expected for the resynthesis step of excision- repair. This uv-stimulated DNA synthesis can be performed by either DNA polymerase II or III and it also requires the uvrA gene product. It requires the four deoxynucleoside triphosphates; but, in contrast to the semiconservative mode, the ATP requirement can be partially satisfied by other nucleoside triphosphates. The ATP-dependent recBC nuclease is not involved. The observed uv-stimulated mode of DNA synthesis may be part of an alternate excision-repair mechanism which supplements or complements DNA-polymerase-I-dependent repair in vivo. (auth)

• Research Organization: Stanford Univ., CA
• NSA Number: NSA-33-012273
• OSTI ID: 4120122
• Country of Publication: United States
• Language: English

Related Subjects

*DNA-- BIOSYNTHESIS
*ESCHERICHIA COLI-- PERMEABILITY
*TOLUENE-- BIOLOGICAL EFFECTS
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
ATP
MUTANTS
N48110 --Life Sciences--Radiation Effects on Biochemicals--In-Vitro
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
NUCLEASES
POLYMERASES
ULTRAVIOLET RADIATION