Effect of mutations in lig and polA on uv-induced strand cutting in a uvrC strain of Escherichia coli

Conference · Tue Dec 31 23:00:00 EST 1974

OSTI ID:4120072

Seeberg, E; Rupp, W D; Hanawalt, P C ^[1]

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1. ed.

In experiments on the kinetics of strand breakage of bacterial DNA in a uvrC mutant, it was shown that the results are similar to those described earlier for the breakage of superinfecting lambda DNA. In the first several minutes after irradiation, more breaks appear in a uvr strain than in the uvrC mutant, but at later times the situation is reversed. The action of the uvrC gene product may be to interfere with the joining by polynucleotide ligase of the break made by the uv endonuclease. (HLW)

• Research Organization: Yale Univ. School of Medicine, New Haven
• NSA Number: NSA-33-012288
• OSTI ID: 4120072
• Country of Publication: United States
• Language: English

Related Subjects

*DNA-- STRAND BREAKS
*ESCHERICHIA COLI-- MUTATIONS
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
GENES
LIGASES
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
NUCLEASES
NUCLEOTIDES
ULTRAVIOLET RADIATION