DARK RECOVERY PHENOMENA IN YEAST. 1. COMPARATIVE EFFECTS WITH VARIOUS INACTIVATING AGENTS
If x irradiated diploid cells of Saccharomyces cerevisiae are stored for 3 to 5 days in the dark in distilled water or in phosphate buffer at 30 deg C, their viability as determined by plating is much greater than if they are plated immediately after irradiation. A similar storage effect was observed with diploid cells treated with ultraviolet radiation or with nitrogen mustard (HN2). No such recovery was observed after heat inactivation or acridine-sensitized photoinactivation. In a given experiment, recovery is characterized by a constant dose-modifying factor, but a wide variation of the DMF is observed for different inactivating agents. Control experiments demonstrate that the recovery does not arise from post-irradiation cell multiplication in the storage medium. The storage procedure is also effective in reducing the fraction of respiratory- deficient colonies after ultraviolet or x irradiation. Recovery occurs in the dark and is partially additive to photoreactivation. Plating on a minimal medium immediately after exposure to x rays or ultraviolet also enhances viability on a constant dosemodification basis. Ploidies higher than diploid also exhibit recovery; on the other hand, haploid yeast does not recover from x-ray inactivation and displays only slight recovery from ultraviolet, or from HN2 inactivation. (auth)
- Research Organization:
- Univ. of Chicago
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-18-008183
- OSTI ID:
- 4113236
- Journal Information:
- Radiation Res., Journal Name: Radiation Res. Vol. Vol: 21
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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Related Subjects
ACRIDINE
BIOLOGY AND MEDICINE
CHROMOSOMES
DISTILLATION
HIGH TEMPERATURE
LIGHT
METABOLISM
MITOSIS
NITROGEN MUSTARD
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHATES
PHOTOCHEMISTRY
QUANTITY RATIO
RADIATION EFFECTS
RECOVERY
SACCHAROMYCES
STORAGE
TEMPERATURE
ULTRAVIOLET RADIATION
VARIATIONS
WATER
X RADIATION
YEASTS
BIOLOGY AND MEDICINE
CHROMOSOMES
DISTILLATION
HIGH TEMPERATURE
LIGHT
METABOLISM
MITOSIS
NITROGEN MUSTARD
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHATES
PHOTOCHEMISTRY
QUANTITY RATIO
RADIATION EFFECTS
RECOVERY
SACCHAROMYCES
STORAGE
TEMPERATURE
ULTRAVIOLET RADIATION
VARIATIONS
WATER
X RADIATION
YEASTS