Recovery of the accumulation ability of thiomethyl-$beta$-galactoside in Escherichia coli after bacteriophage T4 infection

Journal Article · Sun Feb 01 04:00:00 EST 1976 · J. Virol., v. 17, no. 2, pp. 299-306

OSTI ID:4105975

Saijo, N; Okamoto, K

Effects of uv-irradiated and unirradiated T4 phage infection on the $beta$-galactoside accumulation ability in Escherichia coli have been examined by the use of $sup 14$C-labeled thiomethyl-$beta$-galactoside (TMG). Under conditions where a synchronous adsorption of phage takes place, the cellular ability for TMG accumulation is found to be largely inhibited immediately after phage adsorption but it recovers with time to a new level, which is dependent on the multiplicity of infection. When cells are infected with uv-irradiated T4 at the same multiplicity as that of unirradiated phage, the cellular accumulation ability is more severely inhibited and there is no recovery from the inhibition. The recovery process in T4-infected cells is mostly sensitive to puromycin. These results suggest that the initial inhibition of the TMG accumulation ability is probably caused by the adsorption of phage coats, and the subsequent restoration occurs through the action of a phage-directed protein(s). In the recovery process, no new transport system appears to be involved. The restored ability of TMG accumulation is resistant to the action of superinfecting uv phage. However, different mechanisms appear to be operating in T4-infected cells for the establishment of resistance to ghosts and for the recovery from the phage coat- induced inhibition. (auth)

• Research Organization: Osaka Univ.
• NSA Number: NSA-33-017339
• OSTI ID: 4105975
• Journal Information: J. Virol., v. 17, no. 2, pp. 299-306, Journal Name: J. Virol., v. 17, no. 2, pp. 299-306; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

*BACTERIOPHAGES-- BIOLOGICAL RADIATION EFFECTS
*ESCHERICHIA COLI-- BIOLOGICAL RECOVERY
*GLYCOSIDES-- BUILDUP
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
INFECTIVITY
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
PUROMYCIN
ULTRAVIOLET RADIATION