EFFECT OF SOME RADIOPROTECTORS ON THE ENZYMATIC SYSTEM OF MICROSOMES DEGRADATION OF LIVER HEXOBARBITAL

Journal Article · Wed Jan 01 04:00:00 EST 1964 · Biochemical Pharmacology (England)

DOI:https://doi.org/10.1016/0006-2952(64)90078-4· OSTI ID:4095844

Liebecq, C; Bacq, Z M; Thomou, A

Various so-called radioprotectors were tested for their ability to interfere with the metabolism of hexobarbital by sub-cellular fractions of the rat liver in vitro. Cystamine (1.5 x 10/sup -3/ M) and 5-hydroxytryptamine (10/ sup -3/ M) produced a 50% inhibition of the utilization of hexobarbital by liver homogenates prepared in phosphate buffer, freed of the nuclear and mitochondrial fractions, and supplemented with nicotinamide adenine dinucleotide phosphate (NADP) and magnesium ions. Cysteamine and histamine were less effective. The reduction of NADP by added glucose-6phosphate in the presence of the glucose-6- phosphate dehydrogenase of the soluble fraction of the liver homogenate was unaffected by the compounds tested. The reoxidation of the reduced form of NADP by oxygen in the presence of the microsomes was inhibited by cystamine and 5- hydroxytryptamine; this process was initially stimulated and later inhibited by histamine, initially unaffected, and later inhibited by cysteamine. (auth)

• Research Organization: Universite, Liege
• NSA Number: NSA-18-011581
• OSTI ID: 4095844
• Journal Information: Biochemical Pharmacology (England), Journal Name: Biochemical Pharmacology (England) Vol. Vol: 13; ISSN BCPCA
• Country of Publication: Country unknown/Code not available
• Language: French

Related Subjects

ADENINE
AMINES
ANESTHESIA
BIOLOGY AND MEDICINE
CYSTAMINE
DEHYDROGENASES
DRUGS
ENZYMES
GLUCOSE
HISTAMINE
IONS
LIVER
MAGNESIUM
MEA
METABOLISM
MITOCHONDRIA
NUCLEOTIDES
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDATION
OXYGEN
PHOSPHATES
PROTOPLASM
PURINES
RADIATION PROTECTION
RATS
REDUCTION
SEROTONIN
SUGARS
TESTING
TOXICITY