CULTIVATION OF MICROORGANISMS IN HEAVY WATER

Journal Article · Fri Nov 25 04:00:00 EST 1960 · Annals of the New York Academy of Sciences (U.S.)

DOI:https://doi.org/10.1111/j.1749-6632.1960.tb39098.x· OSTI ID:4094152

Crespi, H L; Conrad, S M; Uphaus, R A; Katz, J J

Data are presented on the culture of a variety of algae and other microorganisms in highly deuterated systems. Growth rates for the unicellular green algae Scenedesmus obliquss and Chlorella ralgaris in 99.6% D/sub 2/O were improved so that they approach one-half the rates in H/sub 2/O nutrient media. S. obliquus was also successfully mass cultured in 5-liter units. The blue-green alga Plectonema calothricoides was grown on a continuous laboratory scale in 99.6% D/sub 2/O. A number of other green, blue-green, and red algae and diatoms have been cultured at D/sub 2/O levels from 75 to 99.6%. The bacterium Escherichia coli, the yeast Tonalopsis utilis, and the mold fungus Asperallus niger were grown in fully deuterated media. Protozoa have been cultured at somewhat lower D/sub 2/O concentrationsi Paramecium caudatum routinely in 60% D/ sub 2/O lettuce infusion and Paramecium bursaria in 50% lettuce infusion. In general, algae are best adapted to D/sub 2/O by serial subculyure, 0 to75 to 99 to 99.6% D/sub 2/O, but Chlorella, E. coli, T. utilis, and A. niger can be grown by direct transfer from H/sub 2/O to 99.6% D/sub 2/O nutrient media. (auth)

• Research Organization: Argonne National Lab., Ill.
• Sponsoring Organization: USDOE
• NSA Number: NSA-15-010654
• OSTI ID: 4094152
• Journal Information: Annals of the New York Academy of Sciences (U.S.), Journal Name: Annals of the New York Academy of Sciences (U.S.) Vol. Vol: 84; ISSN ANYAA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ALGAE
ANIMALS
BACTERIA
BIOLOGY AND MEDICINE
FUNGI
HEAVY WATER
REPRODUCTION
YEASTS