The Sensitivity of Ribosomal Functions in Tetrahymena pyriformis to Varying Doses and Dose Rates of Gamma-Radiation

Journal Article · Mon Dec 01 04:00:00 EST 1975 · Radiation Research

DOI:https://doi.org/10.2307/3574240· OSTI ID:4090075

Oleinick, Nancy L.; Rustad, Ronald C.

Polysome content and rate of amino-acid incorporation have been measured in exponentially growing cultures of the ciliate protozoan Tetrahymena pyriformis after doses of 0 to 120 krad of ⁶⁰Co gamma radiation delivered at three different dose rates. The polysome content decreases exponentially with increasing dose at all three dose rates. Curiously enough, the sensitivity to dissociation was lower at the intermediate dose rate than at either the high or low one. Simple target theory analysis of the data indicates a target size for polysome dissociation in the range between a single ribosome and a disome. Amino- acid incorporation is more sensitive to radiation than is polysome content. Data obtained at the highest dose rate suggest that there is a second sensitive target which is considerably smaller than the more sensitive one. (auth)

• Research Organization: Case Western Reserve Univ., Cleveland
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-015027
• OSTI ID: 4090075
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 3 Vol. 64; ISSN 0033-7587
• Publisher: Radiation Research Society
• Country of Publication: United States
• Language: English

Related Subjects

*RADIOSENSITIVITY-- DOSE-RESPONSE RELATIONSHIPS
*RIBOSOMES-- RADIOSENSITIVITY
560114* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Biochemicals--In Animals
BIOLOGICAL RADIATION EFFECTS
COBALT
COMPARATIVE EVALUATIONS
DOSE RATES
GAMMA RADIATION
IRRADIATION
N48140* --Life Sciences--Radiation Effects on Biochemicals-- In Animals
N48530 --Life Sciences--Radiation Effects on Animals--Invertebrates
TETRAHYMENA