Restoration of ultraviolet-induced unscheduled DNA synthesis of xeroderma pigmentosum cells by the concomitant treatment with bacteriophage T4 endonuclease V and HVJ (Sendai virus)

Journal Article · Wed Oct 01 00:00:00 EDT 1975 · Proc. Natl. Acad. Sci. U.S.A., v. 72, no. 10, pp. 4071-4075

DOI:https://doi.org/10.1073/pnas.72.10.4071· OSTI ID:4088107

Tanaka, K; Sekiguchi, M; Okada, Y

Ultraviolet (uv)-induced unscheduled DNA synthesis of xeroderma pigmentosum cells, belonging to complementation groups, A, B, C, D, and E, was restored to the normal level by concomitant treatment of the cells with T4 endonuclease V and uv-inactivated HVJ (Sendai virus). The present results suggest that T4 endonuclease molecules were inserted effectively into the cells by the interaction of HVJ with the cell membranes, the enzyme was functional on human chromosomal DNA which had been damaged by uv irradiation in the viable cells, all the studied groups of xeroderma pigmentosum (variant was not tested) were defective in the first step (incision) of excision repair. (auth)

• Research Organization: Univ., Osaka
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-015038
• OSTI ID: 4088107
• Journal Information: Proc. Natl. Acad. Sci. U.S.A., v. 72, no. 10, pp. 4071-4075, Journal Name: Proc. Natl. Acad. Sci. U.S.A., v. 72, no. 10, pp. 4071-4075; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

*BACTERIOPHAGES
*DNA-- BIOSYNTHESIS
*NUCLEASES
550500* --Biomedical Sciences
Basic Studies--Metabolism
ANIMAL CELLS
N48210* --Life Sciences--Radiation Effects on Cells-- External Source
SKIN DISEASES
ULTRAVIOLET RADIATION
VIRUSES