THE EFFECT OF X-RAY IRRADIATION ON THE CELLS OF EHRLICH'S ASCITIC CARCINOMA AS INVESTIGATED BY RADIOAUTOGRAPHY (in Russian)
A total of 1 x 10/sup 7/ cells of Ehrlich's ascitic carcinoma was administered intraperitoneally to mice of the BALB line. Three days later the mice were subjected to a whole body, x-ray dose of 800 r. Thymidine labeled with tritium (TH/sup 3/ with a specific activity of 3 curies/mmol) was introduced intraperitoneally at a dose of 5 mu C. Smears of ascitic liquid were taken each day on three mice, and radioautographs were prepared in order to determine the uptake of thymidine by the cell as compared to the control. The results show that the transition of the premitotic phase to mitosis (G/sub 2/ yields M transition) is blocked for a period of 10 hours after the irradiation. Then, all the cells undergo a single division, and blocking of the G/sub 2/ phase again takes place. The irradiation had only a slight effect on the synthesis of DNA. The net effects of the irradiation are that cell multiplication is arrested for about one day, and that the distribution of the cell population in various phases changes after the irradiation. These after-effects are of significance in the treatment of cancer of radiotherapy or chemical reagents. (TTT)
- Research Organization:
- Inst. of Chemical Physics, Academy of Sciences, USSR
- NSA Number:
- NSA-18-013482
- OSTI ID:
- 4087951
- Journal Information:
- Doklady Akademii Nauk SSSR (U.S.S.R.) English translation currently published in a number of subject-oriented journals, Vol. Vol: 154; Other Information: Orig. Receipt Date: 31-DEC-64
- Country of Publication:
- Country unknown/Code not available
- Language:
- Russian
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