THE EFFECT OF DEUTERIUM OXIDE ON VIRUS-HOST INTERACTION
The effect of D/sub 2/O on the growth of E. coli and a number of mammalian stable cell lines has been investigated. As the D/sub 2/O concentration was increased, the cells of all the species tested appeared larger, showed an increase in water content and dry weight, and had a slower growth rate. Cytological investigations showed an increase in the number of multinucleated cells and a moderate increase in sudanophilic material. In general, cells grown in D/sub 2/O exhibited cytological characteristics similar to those observed in giant cells produced by irradiation. The effect of D/sub 2/O on bacteriophage replication was examined. The burst size of T5 was somewhat reduced in deuterated E. coli, but the burst size of T7 was significantly increased. These differences might be explained by the fact that whereas the bulk of T5 DNA is derived from the medium, most of the T7 DNA is derived from the host. With increased size of the host, more DNA and protein production might be expected. Whether or not deuteration causes an inhibition of the uptake of various substrates from the medium is under investigation. Studies of the multiplication of poliovirus in deuterated HeLa and monkey kidney cells also showed a marked incresse in burst size. A possible variant of CHAT (attenuated type 1 poliovirus) was isolated from virus progeny grown in deuterated monkey kidney cells. The reason for the increased burst size in deuterated cells is not clear at the present time. Similar increases in burst size were observed in studies of viral replication in x ray treated giant cells. It is possible that the enlargement of cells grown in D/sub 2/O is the underlying factor in the observed increase of poliovirus burst size. (auth)
- Research Organization:
- Wistar Inst. of Anatomy and Biology, Philadelphia
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-15-010655
- OSTI ID:
- 4083932
- Journal Information:
- Annals of the New York Academy of Sciences (U.S.), Journal Name: Annals of the New York Academy of Sciences (U.S.) Vol. Vol: 84; ISSN ANYAA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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