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SPECIFIC HOMOGRAFT TOLERANCE IN LYMPHOID CELLS OF LONG-LIVED RADIATION CHIMERAS

Technical Report ·
OSTI ID:4075112

(LxAF/sub 1/ hybrid mice were exposed to an LD/sub 100/ of x rays (870 n and then injected intravenously with bone marrow cells from genetically foreign (homologous) normal C/sub 3/H strain mice. It was found that the long-term survivors were still chimeras, as evidenced by the presence in the spleen of transplantation immunity isoantigens specific for the marrow donor strain (C/sub 3/H). Immunological reactivity of lymphoid tissue cells from these chimeras was assessed by their capacity, when transferred to lethaily x-irradiated (LxA)F/sub 1/ mice, to cause the rejection of homologous bone marrow. These chimera lymphoid cells brought about the rejection of marrow transplants derived from normal DBA/2 or BALB/c mice strains that are genetically foreign with respect to both the donor and host genotype of the chimeras. However, lymphoid cells from the chimeras were found to be nonreactive. i.e., tolerant. towards marrow transplants of (LxA)F/sub 1/ or of C/sub 3/H origin. Data are presented which indicate strongly the presence of immunologically reactive cells of donor (C/sub 3/H) genotype in the lymphoid tissues of these long-lived chimeras. It is suggested that a state of mutual homograft tolerance exists between the donor and host lymphoid cells in these long-lived radiation chimeras although these cells reject grafts of cells which are homologous to both donor and host type. The implications of these findings as they relate to the phenomenon of actively acquired tolerance and to the clonal selection theory of immunity are discussed. (auth)

Research Organization:
Naval Radiological Defense Lab., San Francisco
NSA Number:
NSA-15-012776
OSTI ID:
4075112
Report Number(s):
USNRDL-TR-499
Country of Publication:
Country unknown/Code not available
Language:
English

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