INHIBITION OF RNA POLYMERASE BY 6-AZAURIDINE TRIPHOSPHATE
Journal Article
·
· Biochim. Biophys. Acta.
ABS>Escherichia coli RNA polymerase was prepared, and the synthetic reaction was assayed by the method of Chamberlin and Berg. Phosphorus-32-labeled uridine triphosphate (UTP) was used as the proximally labeled nucleotide. The pyrophosphate-nucleoside triphosphate exchange reaction dependent on crab- daT(deoxyadenylate and thymidylate) (or daT synthesized by Kornberg's polymerase) was maximal when UTP alone was included in the reaction. Labeled pyrophosphate- UTP exchange was significantly inhibited by aza-UTP. (H.M.G.)
- Research Organization:
- Argonne Cancer Research Hospital, Chicago
- NSA Number:
- NSA-18-015751
- OSTI ID:
- 4063014
- Report Number(s):
- ACRH-20(p.132-5)
- Journal Information:
- Biochim. Biophys. Acta., Journal Name: Biochim. Biophys. Acta. Vol. Vol: 72
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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