LIQUID-SCINTILLATION COUNTING OF C$sup 14$-LABELLED ANIMAL TISSUES AT HIGH EFFICIENCY
Journal Article
·
· Biochemical Journal (England)
BS>The counting of C/sup 14/-labelled substances, soluble and insoluble in the scintillator, at counting efficiencies of 90 and 60% respectively with a single photomultiplier liquidscintillation-counting assembly operating at room temperature is described. The effect of scintillator volume on xylene-soluble sources and sources soluble in aqueous solutions was investigated. Phosphorescence quenching of the scintillator by organic solvents and by oxygen was examined. The importance of phosphorescence of the quartz counting vessel in determining count rates is indicated. A routine method is described for dissolving tissues and proteins by means of potassium hydroxide and a molar solution of Hyamine 10-X in methanol to produce solutions containing 10 mg of dry tissue/ml for liquid-scintillation counting. The use of Hyamine 10-X for blending sources soluble in aqueous solution with scintillator is described. The effect of both the volume of Hyamine 10-X solution used for blending, and of the sample, on counting efficiency was shown with STAC/sup 14/! glycine solutions alone and with STAC/sup 14/!glycine added to tissue solutions. The efficiency of the Hyamine 10-X blending method was demonstrated by measuring the re coveries of added counts,(as STAC/sup 14/! glycine) from tissue and protein solutions. STAC/ sup 14/!Glycine dissolved in these solutions was counted at the same efficiency as in water solution. (auth)
- Research Organization:
- Queen's Univ., Belfast
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-15-014072
- OSTI ID:
- 4062819
- Journal Information:
- Biochemical Journal (England), Journal Name: Biochemical Journal (England) Vol. Vol: 78; ISSN BIJOA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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