ON THE ABNORMAL GERMINATION OF SPORES OF SCHIZOSACCHAROMYCES POMBE AS A RESULT OF X-RAY IRRADIAION

Journal Article · Tue Jan 01 04:00:00 EST 1963 · Compt. Rend. Trav. Lab. Carlsberg

OSTI ID:4056746

Ditlevsen, E; Hartelius, V

It was shown that spores of the fission yeast, S. pombe, which are lethally damaged by x rays are able to germinate and grow to a certain extent. The individual spores were followed in cultures in Buerker-Tuerk chambers until no further development occurred (after two or three days incubation), and their nucleic acid patterns were observed in stained preparations. X-irradiated spores were observed to stop growing at various stages of development, but in a majority of them the cell division mechanism see seemed to be blocked, whereas cytoplasmic growth was able to continue. This caused the appoarance of elongated cells, up to 10 times normal length. Elongated cells seemed to produce the same amourt of cytoplasm as contained in the progeny of a norma1 cell which had multiplied during the same time. The block of cell division was not complete; in many apparertly undivided cells, mitosis was in progress, and in one-third of the cases the development proceeded beyond the point of cell division. The cells underwent cell division, although so late that the daughter cells were also elongated. Furthermore, half of the cells divided in an abnorma1 way by merely forming a cell plnte without the daughter cells separating. Also, purely cytoplasmic changes occurred, manifested by abnormal cell shapes in 5% of the cell s but ordinarily this did not affect mitotic activity. (BBB)

• Research Organization: Carlsberg Lab., Copenhagen
• NSA Number: NSA-18-017511
• OSTI ID: 4056746
• Journal Information: Compt. Rend. Trav. Lab. Carlsberg, Journal Name: Compt. Rend. Trav. Lab. Carlsberg Vol. Vol: 33
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AGE
BIOLOGY AND MEDICINE
CYTOLOGY
DYES
INSTRUMENTS
IRRADIATION
LABORATORY EQUIPMENT
LETHAL DOSE
MICROORGANISMS
MITOSIS
NUCLEIC ACIDS
RADIATION EFFECTS
RADIATION INJURIES
REGENERATION
REPRODUCTION
SPORES
STANDARDS
VARIATIONS
VOLUME
X RADIATION
YEASTS