THE SIGNIFICANCE OF THE SPLEEN FOR THE REACTION OF THE BONE MARROW TO WHOLE- BODY RADIATION (in German)
The uptake of /sup 32/P by bone marrow was studied in vitro in cells from rats that had received x radiation at a rate of 60 r/min, and from cortrol animals. In the normal marrow the uptake of /sup 32/P was linear for the first 20 hr, but the cells from irradiated animals took up /sup 32/P as fast as this only for the first 10 hr. The final total uptake was less than in the normal cells. Bone marrow taken from one to two hr after radiation with 800 r shows, in vitro, a decreased /sup 32/P uptake that is 50 to 60% less than in normal cells. Marrow cells removed from rats 5 hr after radiation have an almost normal uptake rate. In animals where the spleen was removed before exposure to 800 r, uptake was almost the same as in nonsplenectomized animals exposed to 150 r. Splenectomy, as such, had no effect on /sup 32/P uptake 1 day after the operation. The dedecreased /sup 32/P uptake after radiation exposure may be due to decreased DNA synthesis. The smaller inhibitory effect of radiation after splenectomy suggests that DNA synthesis in the marrow cells is directly affected by a radiation injury of the cell, and is indirectiy affected by a regulative function of the spleen. Studies of the electrophoretic hemocytopherogram showed that 3 to 11 hr after exposure to 800 r the granulocyte fraction was absent, but in its place a rapidly moving fraction appeared. These may be immature cells released from the bone marrow, since the granulocyte blood picture was normal 48 hr after the radiation. If the spleen was removed 48 hr before radiation with 800 r, the granulocyte fraction in the hemocytopherogram was unchanged. The results suggest that the spleen regulates release of blood cells from the marrow into the peripheral blood. There appears to be a definite time- and dose-dependent correlation between the decreased synthetic activity of the marrow cells, as measured by /sup 32/P uptake, and increased release of granulocytes from the marrow. Both processes seem to be influenced by the presence of the spleen. (BBB)
- Research Organization:
- Max Planck Institut fuer Biochemie, Munich
- NSA Number:
- NSA-18-017603
- OSTI ID:
- 4049816
- Journal Information:
- Schweiz. Med. Wochschr., Journal Name: Schweiz. Med. Wochschr. Vol. Vol: 93
- Country of Publication:
- Country unknown/Code not available
- Language:
- German
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Related Subjects
ANIMAL CELLS
BIOLOGY AND MEDICINE
BLOOD CELLS
BODY
BONE MARROW
DIAGRAMS
DIFFUSION
DNA
ELECTROPHORESIS
GRANULOCYTES
IRRADIATION
LEUCOCYTES
MEDICINE
METABOLISM
NUCLEIC ACIDS
PHOSPHORUS 32
PHYSIOLOGY
QUANTITY RATIO
RADIATION DOSES
RADIATION INJURIES
RATS
SPLEEN
SURGERY
SUSPENSIONS
VARIATIONS
X RADIATION