Association of immunity and tolerance of host H-2 determinants in irradiated F$sub 1$ hybrid mice reconstituted with bone marrow cells from one parental strain

Journal Article · Fri Aug 01 00:00:00 EDT 1975 · J. Exp. Med., v. 142, no. 2, pp. 321-329

OSTI ID:4038319

Sprent, J; von Boehmer, H; Nabholz, M

Semiallogeneic radiation chimeras were prepared by injecting heavily irradiated F$sub 1$ hybrid mice with bone marrow cells from one parental strain; the bone marrow cells were treated with anti-theta serum and complement to remove T cells and injected in large numbers (2 x 10$sup 7$ cells). The mice survived in excellent health until sacrifice 6 mo later. Thoracic duct cannulation at this stage showed that the mice possessed normal numbers of recirculating lymphocytes. Close to 100 percent of thoracic duct lymphocytes and lymph node cells were shown to be of donor strain origin. The capacity of lymphocytes from the chimeras to respond to host-type determinants was tested in mixed leukocyte culture and in an assay for cell-mediated lympholysis (CML). Mixed leukocyte reactions (MLR) were measured both in vitro and in vivo; tumor cells and phytohemagglutinin-stimulated blast cells were used as target cells for measuring CML. While responding normally to third party determinants, cells from the chimeras gave a definite, though reduced MLR when exposed to host-type determinants. However, this proliferative response to host-type determinants, unlike that to third party determinants, was not associated with differentiation into cytotoxic lymphocytes.

• Research Organization: Basel Inst. for Immunology
• NSA Number: NSA-33-029710
• OSTI ID: 4038319
• Journal Information: J. Exp. Med., v. 142, no. 2, pp. 321-329, Other Information: Orig. Receipt Date: 30-JUN-76
• Country of Publication: United States
• Language: English

Related Subjects

N48520* -Life Sciences-Radiation Effects on Animals- Vertebrates
560152* -Biomedical Sciences
Applied Studies-Radiation Effects-Radiation Effects on Animals-Vertebrates
*BONE MARROW CELLS- BIOLOGICAL RADIATION EFFECTS
*IMMUNE REACTIONS- BIOLOGICAL RADIATION EFFECTS
*RADIATION CHIMERAS- IMMUNE REACTIONS
IMMUNE SERUMS
LEUKOCYTES
LYMPHOCYTES
MICE
PHYTOHEMAGGLUTININ
THYMOCYTES
X RADIATION