Radioimmunoassay for detection of antigen and antibodies to Newcastle disease virus

Journal Article · Thu Apr 01 04:00:00 EST 1976 · J. Immunol., v. 116, no. 4, pp. 1089-1092

OSTI ID:4038273

Spira, G; Silvian, I; Zakay-Rones, Z

When Newcastle disease virus (NDV) is treated with NP-40 and ether a membrane fraction of 150.000 m.w. is obtained. This fraction which is composed of two polypeptides with m.w. of 56,000 and 76,000 was used in a radioimmunoassay (RIA). The assay was developed for both antigen and antibody and was found to be reproducible, specific, and highly sensitive. Titers of 1:51,200 were determined by RIA as compared to 1 : 4 by agar gel diffusion and 1 : 200 by hemaglutination inhibition (HI). As little as 5 ng of viral protein were detected by RIA inhibition technique. Labeled antigen could be stored in the presence of serum, KCI and Triton X-100 at -20$sup 0$C for as long as 6 weeks and retained similar reactivity as fresh reagent.

• Research Organization: Hebrew Univ., Jerusalem
• NSA Number: NSA-33-029944
• OSTI ID: 4038273
• Journal Information: J. Immunol., v. 116, no. 4, pp. 1089-1092, Journal Name: J. Immunol., v. 116, no. 4, pp. 1089-1092; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

*ANTIBODIES-- RADIOIMMUNOASSAY
*ANTIGENS-- RADIOIMMUNOASSAY
*VIRUSES
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