RESTORATION OF VIABILITY OF X-IRRADIATED L-STRAIN CELLS BY ISOLOGOUS SUBCELLULAR FRACTIONS

Journal Article · Thu Aug 01 04:00:00 EDT 1963 · Intern. J. Radiation Biol.

DOI:https://doi.org/10.1080/09553006314550961· OSTI ID:4036487

Petrovic, D; Miletic, B; Brdar, B

L-cells were homogenized, and nuclei, mitochondria, and microsomes were isolated afterwards. These subcellular fractions were added to cells irradiated with 500 r. In many experiments the irradiated and treated cells gave a considerably higher number of colonles than the irradiated nontreated cells, and the colonies of treated cells were much larger. Restoration was considerable on treating the cells with the nuclear fraction. Mitochondria and microsomes were not so active in restoring from the killing effect of irradiation. The addition of subcellular fractions to nonirradiated cells had some effect on the plating efficiency of cells and the appearance of colonies, but not so great as to explain the restorative effect as a phenomenon of increased plating efficiency. In the subcellular fractions intact or viable cells were not detectable. ln some cases the addition of subcellular fractions produced no effect. (auth)

• Research Organization: Institut Rudjer Boskovic, Zagreb
• Sponsoring Organization: USDOE
• NSA Number: NSA-18-019783
• OSTI ID: 4036487
• Journal Information: Intern. J. Radiation Biol., Journal Name: Intern. J. Radiation Biol. Vol. Vol: 7
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BIOLOGY AND MEDICINE
CELL CULTURES
CELL NUCLEI
CYTOLOGY
EFFICIENCY
ENZYMES
IRRADIATION
LETHAL DOSE
MITOCHONDRIA
NUCLEIC ACIDS
PROTOPLASM
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
RECOVERY
REGENERATION
SURVIVAL TIME
SUSPENSIONS
VARIATIONS
X RADIATION