Acid-$beta$-glycerophosphatase reaction products in the central nervous system mitochondria following x-ray irradiation
A survey of the literature to date on the enzyme histochemistry of intracellular organelles has not yielded any reference to the presence of acid phosphatase reaction products in the mammalian mitochondria of the central nervous system. A combination of Gomori's acid phosphatase method, however, with standard electron microscopy has disclosed the presence of enzyme reaction products in the mitochondria of the central nervous system of rats from 2 hr to 22 weeks after x-ray irradiation, as well as in a cerebral biopsy performed on a patient affected by Huntington's chorea. No enzyme reaction products, on the other hand, were observed in serial sections that had been incubated in substrates either containing sodium fluoride or lacking in $beta$- glycerophosphate. The abnormal mitochondrial enzyme reaction (chemical lesion) is considered to be the consequence of the pathologic process affecting the ultrastructural-chemical organization of the organelle. (auth)
- Research Organization:
- New York State Psychiatric Inst., NY
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-33-026273
- OSTI ID:
- 4032061
- Journal Information:
- J. Histochem. Cytochem., v. 23, no. 6, pp. 402-410, Other Information: Orig. Receipt Date: 30-JUN-76
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560152* -Biomedical Sciences
Applied Studies-Radiation Effects-Radiation Effects on Animals-Vertebrates
*ACID PHOSPHATASE- BIOLOGICAL RADIATION EFFECTS
*CENTRAL NERVOUS SYSTEM- BIOLOGICAL RADIATION EFFECTS
*MITOCHONDRIA- BIOLOGICAL RADIATION EFFECTS
LOCAL IRRADIATION
PHYSIOLOGY
RATS
SPINAL CORD
X RADIATION