EFFECTS OF MASSIVE DOSES OF GAMMA RADIATION (120,000r) ON THE CHROMOSOMES OF SUCCESSIVE GENERATIONS OF HYMENOLEPIS DIMINUTA
The cumulative effects of radiation on the chromosomes of successive generations of Hymenolepis diminuta were studied. The cysticercoid stage of the worms in flour beetles (Tribolium confusum) was subjected to 15,000 r gamma radiation. These irradiated cysts were fed to rats, where they developed into mature worms in the intestine. The eggs from these worms were fed to beetles, and the cysticercoids were subjected again to a dose of 15,000 r. The process was repeated for 8 successive generations, thereby building up the cumulative dose to 120,000 r. Four worms irradiated in this manner were separated and their descendants were further traced down through single worm infection up to 4 generations, thus giving rise to 4 distinct groups. Chromosomes in meioses and mitoses of 19 adult worms belonging to the fourth generatlon were studied by aceto-orcein squashes of selected pontions of the strobila. Three normal worms served as control. Chromosome lengths of each worm were determined from average lengths of chromosomes in mitotic metaphases. Chromosomes of irradiated worms appeared to have been altered in lengths, numbers, and morphology. Variations in chromosome number, above and below the diploid numbers of 12 were noted. Chromosome bridges and chromosomes exceeding normal length were seen in a few cells. On the basis of changes in chromosome lengths and structure, 4 different cytological strains were produced by the cumulative effects of radiation. Possible uses of these new strains in further cytological studies are suggested. (Dissertation Abstr., 24: No. 8, Feb. 1964)
- Research Organization:
- Originating Research Org. not identified
- NSA Number:
- NSA-18-019752
- OSTI ID:
- 4022541
- Resource Relation:
- Other Information: Thesis. Orig. Receipt Date: 31-DEC-64
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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