Oxidative stress in lake sturgeon orally exposed to 2,3,7,8-tetrachlorodibenzofuran
- Univ. of Manitoba, Winnipeg, Manitoba (Canada). Dept. of Zoology
- Department of Fisheries and Oceans, Winnipeg, Manitoba (Canada)
Juvenile lake sturgeon were orally dosed with gelatin containing nominal concentrations of 0, 0.16 or 1.6 ng 2,3,7,8-tetrachlorodibenzofuran (TCDF)/Kg fish weight. Liver, kidney and blood were collected 10 and 27 days after exposure. Phase 1 (mixed function oxidase MFO) and Phase 2 (glucuronyltransferase) enzyme activities were determined in liver. In addition, concentrations of nonenzymatic (ascorbic acid, tocopherol, retinoids) and enzymatic (superoxide dismutase, catalase, glutathione peroxidase) antioxidant parameters were quantified in liver and kidney. TCDF concentrations were elevated in liver and kidney of both dose groups at 10 and 27 days. However, lower tissue concentrations of TCDF at 27 days compared with 10 day exposures, as well as the presence of polar metabolites in bile, suggest rapid metabolism and clearance of the contaminant. Phase 1 and Phase 2 enzyme activities were induced in a dose dependent manner with activities at the two sample periods reflecting the different TCDF concentrations. Indicators of oxidative stress responded similarly.
- OSTI ID:
- 40091
- Report Number(s):
- CONF-9410273-; TRN: IM9520%%286
- Resource Relation:
- Conference: 15. annual meeting of the Society of Environmental Toxicology and Chemistry (SETAC), Denver, CO (United States), 30 Oct - 3 Nov 1994; Other Information: PBD: 1994; Related Information: Is Part Of Society of Environmental Toxicology and Chemistry 15th annual meeting: Abstract book. Ecological risk: Science, policy, law, and perception; PB: 286 p.
- Country of Publication:
- United States
- Language:
- English
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