Oxidative stress in lake sturgeon orally exposed to 2,3,7,8-tetrachlorodibenzofuran

Conference · Sat Dec 31 04:00:00 EST 1994

OSTI ID:40091

Palace, V P; Dick, T A ^[1]; Brown, S B; Baron, C L ^[2]; Klaverkamp, J F ^[1]

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1. Univ. of Manitoba, Winnipeg, Manitoba (Canada). Dept. of Zoology
2. Department of Fisheries and Oceans, Winnipeg, Manitoba (Canada)

Juvenile lake sturgeon were orally dosed with gelatin containing nominal concentrations of 0, 0.16 or 1.6 ng 2,3,7,8-tetrachlorodibenzofuran (TCDF)/Kg fish weight. Liver, kidney and blood were collected 10 and 27 days after exposure. Phase 1 (mixed function oxidase MFO) and Phase 2 (glucuronyltransferase) enzyme activities were determined in liver. In addition, concentrations of nonenzymatic (ascorbic acid, tocopherol, retinoids) and enzymatic (superoxide dismutase, catalase, glutathione peroxidase) antioxidant parameters were quantified in liver and kidney. TCDF concentrations were elevated in liver and kidney of both dose groups at 10 and 27 days. However, lower tissue concentrations of TCDF at 27 days compared with 10 day exposures, as well as the presence of polar metabolites in bile, suggest rapid metabolism and clearance of the contaminant. Phase 1 and Phase 2 enzyme activities were induced in a dose dependent manner with activities at the two sample periods reflecting the different TCDF concentrations. Indicators of oxidative stress responded similarly.

• OSTI ID: 40091
• Report Number(s): CONF-9410273--
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BENZOFURANS
BIOLOGICAL STRESS
DOSE-RESPONSE RELATIONSHIPS
FISHES
TISSUE DISTRIBUTION
TOXICITY
WATER POLLUTION