Protonation/deprotonation processes of primary products in x-irradiated cytosine derivatives: EPR and ENDOR studies at 10 K
- Univ. of Oslo (Norway). Dept. of Physics
- Georgia State Univ., Atlanta, GA (United States). Dept. of Physics and Astronomy
- East Tennessee State Univ., Johnson City, TN (United States). Dept. of Physics
Recently, evidence has accumulated that cytosine is the initial electron trap in irradiated DNA. It has previously been observed that the protonation/deprotonation mechanisms and sites of the pristine purine/pyrimidine electron gain and loss centers depend upon the matrix. Local variations as e.g. water of crystallization, hydrogen bond pattern, initial protonation state of the cytosine bases, and associated counter ions may be important in the selection of reaction pathways following the initial ionization event. In response to this increased interest in the primary radical production in irradiated cytosine, five different crystalline cytosine derivatives have been investigated at 10 K following X-irradiation exposure to doses between 0.8 and 30 kGy. The techniques used are K-band EPR, ENDOR, and ENDOR-induced EPR (FSE) spectroscopy. The systems studied are: cytosine monohydrate (Cm); cytosine-HCI (C:HCl); cytidine (CR); deoxycytidine:HCI (CdR:HCl); and 2{prime}-deoxycytidine 5{prime}-monophosphate (5{prime}dCMP).
- OSTI ID:
- 400720
- Report Number(s):
- CONF-9410280--; ISBN 0-935470-90-5
- Country of Publication:
- United States
- Language:
- English
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