Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on early life stage development of the fathead minnow (Pimephales promelas)
- Univ. of Utrecht (Netherlands)
Eggs of fathead minnow were exposed to graded concentrations (0.1--100 ppt) of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at 24 C for 5 days. Exposure started about one hour after fertilization. The medium was refreshed daily. At day 6 (one day posthatching), body lengths were measured. A dose-dependent retardation of growth (from 1 to 30%) was observed in the range from 0.1 to 20 ppt TCDD. At higher concentrations, growth reduction remained the same but mortality still increased. Microscopic observations of the TCDD-exposed embryos revealed a number of deviations from the normal early development. Initial changes consisted of congestion of the blood flow in the caudal tail area and in the yolk sac vascular complex, followed by hemorrhages. At a later stage, pericardial edema and generalized edema were observed. In addition the embryos revealed, anaemia, decreased amounts of dermal and retinal melanin, shortened mandibles, degeneration of heart tissue, absence of swim bladder, decreased utilization of yolk sac material and a curved chords. The results indicate that embryonic development of the fathead minnow is very sensitive to TCDD. It might prove a useful model for further embryotoxic studies.
- OSTI ID:
- 40044
- Report Number(s):
- CONF-9410273-; TRN: IM9520%%239
- Resource Relation:
- Conference: 15. annual meeting of the Society of Environmental Toxicology and Chemistry (SETAC), Denver, CO (United States), 30 Oct - 3 Nov 1994; Other Information: PBD: 1994; Related Information: Is Part Of Society of Environmental Toxicology and Chemistry 15th annual meeting: Abstract book. Ecological risk: Science, policy, law, and perception; PB: 286 p.
- Country of Publication:
- United States
- Language:
- English
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