2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) toxicity at three stages of lake trout egg development
- Univ. of Wisconsin, Madison, WI (United States)
Although injection of newly fertilized eggs has proven useful for exposure of rainbow trout early life stages to polyhalogenated aromatic hydrocarbons (PHAHs), it has been unsuccessful in lake trout due to injection-related egg mortality. Waterborne exposure of lake trout eggs to vehicle or graded doses of TCDD within 48 hours after fertilization and injection of vehicle or graded doses of TCDD 3 weeks and < 1 week pre-hatch were used to determine the success of egg injection after eye-up and characterize the toxicity of TCDD at later egg stages. A smaller percentage of mortality in later injection groups was associated with signs of TCDD toxicity that have been seen previously (yolk sac edema, hemorrhages, craniofacial malformations and hatching mortality) and the lethal potency of TCDD was reduced. LD50s calculated using probit analysis were 75 pg/g for exposure as newly fertilized eggs and 196 pg/g for injection 3 weeks pre-hatch. The highest dose of 269 pg/g resulted in 30% mortality when injected < 1 week pre-hatch. Because injection-related mortality was very low, with an average of 1% of eggs lost before hatch, injection of eggs after eye-up may be useful for determining the relative potencies of PHAH congeners in lake trout early life stages.
- OSTI ID:
- 40041
- Report Number(s):
- CONF-9410273--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Interactions of polychlorinated biphenyls (PCBs) {number_sign}126, {number_sign}105 and {number_sign}118 in rainbow trout early life stages
Potency of PCDD, PCDF, PCB mixture to produce salmonid developmental mortality compared to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)