Chemical analysis and in vitro EROD activities in extracts from the American oyster
- Texas A and M Univ., College Station, TX (United States)
Chemical residue analysis and EROD induction activities were measured in extracts from three groups of American oysters (Crassostrea virginica) after uptake-depuration experiments conduct in Galveston Bay, Texas. Levels of polynuclear aromatic hydrocarbons (PAHs), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/PCD) were determined by HRGC/HRMS and HRGC/HRMS while congener specific polychlorinated biphenyls (PCBs) were determined by HRGC/EC Induction of ethoxyresorufin O-deethylase (EROD) activity by sample extracts were determined in rat hepatoma H4IIE cells. Oysters from Hanna Reef (HR), a relatively clean site in Galveston Bay, were transplanted to a contaminated site in the Houston Ship Channel (SC) area for 48 days. After this exposure period, the remaining oysters were retransplanted to their original (HR) site to study the depuration of the toxic compounds benzo[a]pyrene (BAP), dibenzo[a,h]anthracene (DBA), PCB77, PCB126, PCB105, PCB118, 2,3,7,8-TCDD, and 2,3,7,8-TCDF concentrations were measured during the uptake experiment and used to estimate the corresponding Induction/Toxic TCDD equivalents (I-TEQS) which ranged from 0.13 to 0.499 ng/g. The results demonstrate that BAP and DBA are major contributors to the TCDD induction equivalents (55--76%) of calculated I-TEC compared to PCBs (16--38%) and TCDD/TCDF (5--10%). The bioassay results gave I-TEQs approximately 10 fold higher than the calculated values suggesting that other compounds not detected by HRGC/HRMS, LRMS or ECD were present in the samples and are active as inducers of EROD activity.
- OSTI ID:
- 392317
- Report Number(s):
- CONF-9511137--; ISBN 1-880611-03-1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ANTHRACENE
BAYS
BENZOFURANS
BENZOPYRENE
BIOLOGICAL INDICATORS
BIOLOGICAL MARKERS
BIOLOGICAL STRESS
DIOXIN
ENZYME ACTIVITY
ENZYME INDUCTION
OXIDOREDUCTASES
OYSTERS
POLYCHLORINATED BIPHENYLS
POLYCYCLIC AROMATIC HYDROCARBONS
TEXAS
TOXICITY
WATER POLLUTION