Detection of complex genetic alterations in human glioblastoma multiforme using comparative genomic hybridization

Journal Article · Mon Jan 01 04:00:00 EST 1996 · Journal of Neuropathology and Experimental Neurology

DOI:https://doi.org/10.1097/00005072-199601000-00008· OSTI ID:391301

Schlegel, J; Stumm, G ^[1]; Scherthan, H; Arens, N ^[2]

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1. Universitaet Marburg (Germany)
2. Universitaet Kaiderlautern (Germany); and others

The aim of the present study was to detect complex genetic alterations in human glioblastoma multiforme (GBM) by comparative genomic in situ hybridization (CGH). Of the 24 GBM that were examined, increased fluorescence intensities indicating chromosomal polysomy of chromosome 7 and gene amplification at chromosome 7p were found in 42% of the tumors. In addition, signal enhancement of chromosome 19 was present in 29% and at 12q13-15 in 21% of the tumors. We also detected reduction of fluorescence intensities indicating gross deletions on chromosomes 10 (58%), 9p (46%), and 13 (29%). There was a close correlation of CGH results when compared with Southern analysis of the EGFR gene localized on chromosome 7 and loss of heterozygosity detection of chromosome 9 and 10 by microsatellite PCR. A close correlation was also observed between copy number changes of chromosome 7 and deletions of chromosome 10. Amplification of chromosome 12q and deletions of chromosomes 9p and 13 seemed to be complementary in the tumors investigated in the present study. 44 refs., 3 figs., 1 tab.

• Sponsoring Organization: USDOE
• OSTI ID: 391301
• Journal Information: Journal of Neuropathology and Experimental Neurology, Journal Name: Journal of Neuropathology and Experimental Neurology Journal Issue: 1 Vol. 55; ISSN JNENAD; ISSN 0022-3069
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CHROMOSOMAL ABERRATIONS
DETECTION
DNA HYBRIDIZATION
GENE AMPLIFICATION
GENE MUTATIONS
GENES
GENETIC MAPPING
GENOME MUTATIONS
GLIOMAS
HUMAN CHROMOSOMES
POLYMERASE CHAIN REACTION
STATISTICS