Fluorescence dynamics of biological systems using synchrotron radiation
- Laboratory for Fluorescence Dynamics, Department of Physics, University of Illinois at Urbana-Champaign, 1110 West Green Street, Urbana, IL, 61801 (United States)
- University of Illinois at Urbana-Champaign, Department of Biochemistry, 1209 W. California St., Urbana, IL 61801 (United States)
- School of Pharmacy, University of Wisconsin-Madison, 425 N. Charter Street, Madison, WI 53706 (United States)
- Department of Biochemestry and Biophysics, University of Hawaii, 1960 East-West Road, Honolulu, Hawaii 9682 (United States)
- Synchrotron Radiation Center, U. Wisconsin-Madison, 3731 Schneider Dr., Stoughton, WI 53589 (United States)
A beamline for time-resolved fluorescence spectroscopy of biological systems is under construction at the Synchrotron Radiation Center. The fluorometer, operating in the frequency domain, will take advantage of the time structure of the synchrotron radiation light pulses to determine fluorescence lifetimes. Using frequency-domain techniques, the instrument can achieve an ultimate time resolution on the order of picoseconds. Preliminary experiments have shown that reducing the intensity of one of the fifteen electron bunches in the storage ring allows measurement of harmonic frequencies equivalent to the single-bunch mode. This mode of operation of the synchrotron significantly extends the range of lifetimes that can be measured. The wavelength range (encompassing the visible and ultraviolet), the range of measurable lifetimes, and the stability and reproducibility of the storage ring pulses should make this beamline a versatile tool for the investigation of the complex fluorescence decay of biological systems. {copyright} {ital 1996 American Institute of Physics.}
- OSTI ID:
- 390555
- Report Number(s):
- CONF-9510119-; ISSN 0034-6748; TRN: 96:027979
- Journal Information:
- Review of Scientific Instruments, Vol. 67, Issue 9; Conference: SRI `95: synchrotron radiation instrumentation symposium and the 7. users meeting for the advanced photon source (APS), Argonne, IL (United States), 16-20 Oct 1995; Other Information: PBD: Sep 1996
- Country of Publication:
- United States
- Language:
- English
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