Effects of ozone on the epithelial and inflammatory responses in the airways: Role of tumor necrosis factor

Journal Article · Wed Nov 01 04:00:00 EST 1995 · Journal of Toxicology and Environmental Health

DOI:https://doi.org/10.1080/15287399509532039· OSTI ID:387351

Young, C; Bhalla, D K ^[1]

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1. Univ. of California, Irvine, CA (United States)

the possibility that tumor necrosis factor {alpha} (TNF) plays a role in the increased airway permeability and an inflammatory response following an acute ozone (O{sub 3}) exposure was investigated. Male Sprague-Dawley rats were injected, intraperitoneally, with either rabbit anti-mouse antibody to TNF (anti-TNF) or preimmune rabbit serum, 2 h before a 3-h exposure to O{sub 3} or purified air. Permeability, as determined by [{sup 99m}Ic] diethylenetriamine pentaacetate (DTPA) transport, total protein and albumin concentrations in the bronchoalveolar lavage (BAL), and the inflammatory cell response in the BAL were assessed 10 h later. The O{sub 4}-exposed group injected with anti-TNF showed a significant decrease in permeability to DTPA in comparison to the O{sub 3}-exposed group injected with preimmune rabbit serum. There was no difference between the anti-TNF group and the purified air group. In contrast, the total protein and albumin levels in the BAL were significantly greater in both of the O{sub 3}-exposed groups than in the purified air group.The concentrations of protein and albumin in the anti-TNF group did, however, show an attenuating trend when compared to the preimmune O{sub 4}-exposed group. The polymorphonuclear leukocytes (PMNs) in BAL of the anti-TNF group also showed an attenuating trend when compared to the preimmune O{sub 4}-exposed group, but both of these O{sub 3}-exposed groups were significantly greater than the purified air group. Lung sections stained with naphthol AS-D chloroacetate esterase showed an increase in the number of stained PMNs in the anti-TNF group in comparison to the preimmune O{sub 4}- and air-exposed groups. These data suggest that TNF plays a role in the increase in tracheal permeability as determined by DTPA transport, while the contributing role that TNF plays in bronchoalveolar permeability and the inflammatory response seen following an acute exposure to 0.8 ppm O{sub 3} is less evident. 35 refs., 3 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 387351
• Journal Information: Journal of Toxicology and Environmental Health, Journal Name: Journal of Toxicology and Environmental Health Journal Issue: 3 Vol. 46; ISSN 0098-4108; ISSN JTEHD6
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ACUTE EXPOSURE
BIOLOGICAL EFFECTS
INFLAMMATION
OZONE
PERMEABILITY
RESPIRATORY TRACT CELLS
RESPONSE MODIFYING FACTORS