Induction of DNA-protein cross-links in developing embryos of the purple sea urchin, Strongylocentrotus purpuratus
- Univ. of California, Davis, CA (United States). Bodega Marine Lab.
- Lawrence Berkeley Lab., Berkeley, CA (United States)
Exposure to environmental agents during embryonic development may result in DNA-protein cross-linking (DPC), as has been demonstrated for mammalian cell lines. In the latter, formation of DPC`s upon exposure to a wide variety of agents, including some metals, has been observed. To determine whether DPCs could be detected in the sea urchin embryo during development, the authors adapted a mammalian cell assay utilizing potassium-SDS precipitation and a DNA fluorochrome to quantify relative amounts of free and protein-bound DNA. Sea urchin embryos exposed to a known DPC agent, nickel, through gastrulation exhibited a dose-dependent increase in DPCs, as well as an increase in developmental abnormalities. Morphological studies demonstrated that stage-specific exposure to Ni prior to gastrulation resulted in similar levels of abnormal pluteus larval development as compared to embryos exposed through gastrulation. Sea urchin embryos exhibit temporal differences in DNA transcription and gene expression during development, and these could be affected by modifications in DNA-protein interactions. Therefore, the authors are investigating the hypothesis that the similarities in morphological responses observed may relate to susceptibility of a critical stage of development.
- OSTI ID:
- 37512
- Report Number(s):
- CONF-9410273-; TRN: IM9519%%436
- Resource Relation:
- Conference: 15. annual meeting of the Society of Environmental Toxicology and Chemistry (SETAC), Denver, CO (United States), 30 Oct - 3 Nov 1994; Other Information: PBD: 1994; Related Information: Is Part Of Society of Environmental Toxicology and Chemistry 15th annual meeting: Abstract book. Ecological risk: Science, policy, law, and perception; PB: 286 p.
- Country of Publication:
- United States
- Language:
- English
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