Teratogenic and genotoxic responses of larval Chironomus (Diptera) to contaminated sediments
Conference
·
OSTI ID:37507
- Univ. of Windsor, Ontario (Canada)
Sediment-associated contaminants can produce developmental or genotoxic stresses independently of their cytotoxic effects. In the laboratory, the authors exposed Chironomus larvae to mixtures of polluted (either Detroit R., MI, or cadmium or benzo-[a]-pyrene-spiked) sediment diluted with uncontaminated, formulated sediment. Second-instar Chironomus nr. salinarius were grown to 4th star in water filled 1-L jars containing 300 mL of contaminated:formulated sediment mixture in ratios of 1:0, 1:1, 1:3, 1:7, 1:15 or 0:1. Surviving larvae were preserved in Carnoy`s solution. Each larva`s head was slide-mounted and examined for deformities of the mentum. Polytene chromosome preparations were made from salivary glands of the same animals using acid fuschin staining and examined for reduced relative size of the nuclear organizer (NO) indicative of inhibition of RNA synthesis activity. Incidence of chironomid deformities from control (0:1) sediments ({plus_minus}I SE) was 7.9 {plus_minus} 1.6% (N = 268): 4.0 {plus_minus} 1.5% of 178 control larvae examined displayed NO reduction. Incidence of mentum deformities and of NO reduction increased linearly with each doubling of Detroit R. concentration at 1:0 for deformities; 12.2 {plus_minus} 3.5% (N = 149) for NO reductions. Reduction of NO in a larva was unrelated to mentum condition, indicating that these are independent responses to contaminant stress. Equivalent results were obtained for exposure to single-compound sediments. This is the first documentation of controlled dose-response effects of contaminants on chironomid deformities.
- OSTI ID:
- 37507
- Report Number(s):
- CONF-9410273--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Spatial variation in incidence of mouthpart deformities in larval chironomids (Diptera) from western Lake Erie
Use of chironomids (Diptera: Chironomidae) as bioindicators of contaminant stress: Biochemical, chromosomal and developmental measures
In situ bioassay using Chironomus riparius: An intermediate between laboratory and field sediment quality assessments
Conference
·
Sat Dec 30 23:00:00 EST 1995
·
OSTI ID:367448
Use of chironomids (Diptera: Chironomidae) as bioindicators of contaminant stress: Biochemical, chromosomal and developmental measures
Conference
·
Sat Dec 30 23:00:00 EST 1995
·
OSTI ID:458288
In situ bioassay using Chironomus riparius: An intermediate between laboratory and field sediment quality assessments
Conference
·
Sat Dec 30 23:00:00 EST 1995
·
OSTI ID:367438