Storage duration and the toxicity of spiked sediment to Rhepoxynius abronius
- Environmental Protection Agency, Newport, OR (United States)
- Battelle Marine Lab., Sequim, WA (United States)
Toxicity tests with spiked sediment are often used in research and regulatory programs. Spiked sediment is usually stored prior to testing, although the effects of storage on contaminant bioavailability and toxicity are poorly understood. The authors spiked a clean sand (97.0% sand, 3.0% silt + clay, 0.2%organic carbon) and a muddy sand (75.8% sand, 24.2% silt + clay, 0.6% organic carbon) with fluoranthene, stored them for 13, 27, 41, 55, 69, 83, 121, and 170 d and tested them with standard 10 d toxicity tests with the infaunal marine amphipod, Rhepoxynius abronius. Both sediments were spiked at nine concentrations of fluoranthene ranging from 0.0 to 23.2 mg/g OC and stored at 4 C until tested. The concentration of fluoranthene in the sediment and interstitial water was determined on samples taken on each test data. The LC50s for fluoranthene in the muddy sand were similar for all storage times, 5.8--7.9 mg/g OC. The LC50 in the clean sand was 6.3 mg/g OC after 13 d of storage, fairly stable at 9.2--11.5 mg/g OC during 27--83 d of storage, and increased to 22.3 and >23.2 mg/g OC after 121 and 180 d of storage, respectively. The results suggest that the bioavailability of fluoranthene in spiked muddy sand remains nearly constant from 13 to 170 d of storage. The higher toxicity in clean sand on day 13 indicates greater bioavailability probably because fluoranthene partitioning had not reached equilibrium. Reduced bioavailability after 121 d may have been caused by disequilibrium or unknown storage effects. These data suggest that spiked sediment toxicity tests with polynuclear aromatic hydrocarbons conducted before 27 d or after 83 d of storage may give misleading results.
- OSTI ID:
- 372559
- Report Number(s):
- CONF-9511137-; ISBN 1-880611-03-1; TRN: IM9641%%636
- Resource Relation:
- Conference: 2. Society of Environmental Toxicology and Chemistry (SETAC) world conference, Vancouver (Canada), 5-9 Nov 1995; Other Information: PBD: 1995; Related Information: Is Part Of Second SETAC world congress (16. annual meeting): Abstract book. Global environmental protection: Science, politics, and common sense; PB: 378 p.
- Country of Publication:
- United States
- Language:
- English
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