Recombinant synthesis and solution characterization of the protein, apolipophorin-III: An amphipathec biopolymer for the removal of foulants from aqueous systems
Conference
·
OSTI ID:370857
- Univ. of Southern Mississippi, Hattiesburg, MS (United States)
The amphipathic biopolymer, apolipophorin-III, functions in the tobacco hornworm to shuttle hydrophobic and amphipathic materials through the aqueous hemolymph of the insect. The function of this protein in vivo suggests that it has the potential to act as a remediative agent for contaminated waters. The protein has been recombinantly produced and purified. Apo-III has been shown to partition to an oil water interface in a concentration dependent manner. In addition, this protein has been shown to sparingly phase transfer hydrocarbons into aqueous solutions. The association of Apo-III with a hydrophobic, fluorescent probe exhibits dramatic changes in response to pH suggesting a trigger mechanism for the capture and release of foulants in water. Site specific modification of the protein is currently underway in an effort to improve the sequestration and emulsification behavior of apo-III.
- OSTI ID:
- 370857
- Report Number(s):
- CONF-960376--
- Country of Publication:
- United States
- Language:
- English
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