Use of synchronous fluorescence spectrometry for detecting conjugated monohydroxypyrene in fish bile and Dungeness crab haemolymph
- Simon Fraser Univ., Burnaby, British Columbia (Canada)
- Inst. of Ocean Sciences, Sidney, British Columbia (Canada). Ocean Chemistry Div.
A Synchronous Fluorescence Spectrometric method (SFS) was used by F. Ariese et al. as a rapid screening method to determine the extent of exposure and uptake of polycyclic aromatic hydrocarbons (PAHs) by fish. This technique makes use of the presence of the major pyrene metabolite, conjugated monohydroxypyrene (CMHP) in the bile of fish as an indicator of PAH exposure and contamination. In the present studies the authors applied this method as part of a biomonitoring study for assessing fish exposure to PAH in Kitimat Arm, British Columbia. They also modified this method and adapted it to screen for CMHP in the haemolymph of crabs. English sole (Parophrys vetulus), Chinook salmon (Oncorhynchus tshawytscha) and Dungeness crabs (Cancer magister) were caught in different areas of Kitimat Arm. Additionally fish and crab tissue samples were collected from reference sites assumed to be uncontaminated. Results show a spatial trend in the levels of CMHP in fish bile and crab haemolymph of Kitimat Arm. The highest levels were found in bile and haemolymph samples collected in the inner Harbor near the main source of PAH contamination. Levels of CMHP in bile and haemolymph decrease with distance down the arm from the source. Samples from Kitimat arm show high levels of PAH contamination compared with samples taken from clean reference sites.
- OSTI ID:
- 367458
- Report Number(s):
- CONF-9511137--; ISBN 1-880611-03-1
- Country of Publication:
- United States
- Language:
- English
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