Genetic analysis of biodegradation of tetralin by a Sphingomonas strain

Journal Article · Thu Apr 01 00:00:00 EST 1999 · Applied and Environmental Microbiology

OSTI ID:343587

Hernaez, M J; Santero, E ^[1]; Reineke, W ^[2]

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1. Univ. de Sevilla (Spain). Dept. de Genetica
2. Bergische Univ.-Gesamthochschule Wuppertal (Germany)

Tetralin (1,2,3,4-tetrahydronaphthalene) is produced for industrial purposes from naphthalene by catalytic hydrogenation or from anthracene by cracking. A strain designated TFA which very efficiently utilizes tetralin has been isolated from the Rhine river. The strain has been identified as Sphingomonas macrogoltabidus, based on 16S rDNA sequence similarity. Genetic analysis of tetralin biodegradation has been performed by insertion mutagenesis and by physical analysis and analysis of complementation between the mutants. The genes involved in tetralin utilization are clustered in a region of 9 kb, comprising at least five genes grouped in two divergently transcribed operons.

• OSTI ID: 343587
• Journal Information: Applied and Environmental Microbiology, Vol. 65, Issue 4; Other Information: PBD: Apr 1999
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
BIODEGRADATION
TETRALIN
REMEDIAL ACTION
DNA SEQUENCING
GENES
GENE OPERONS