Video-rate confocal scanning laser microscope for imaging human tissues {ital in vivo}
- Department of Dermatology, Wellman Laboratories, Massachusetts General Hospital, Bartlett Hall Ext. 630, 50 Blossom Street, Boston, Massachusetts 02114 (United States)
We have built a video-rate confocal scanning laser microscope for reflectance imaging of human skin and oral mucosa {ital in vivo}. Design and imaging parameters were determined for optimum resolution and contrast. Mechanical skin-holding fixtures and oral tissue clamps were made for stable objective lens-to-tissue contact such that gross tissue motion relative to the microscope was minimized. Confocal imaging was possible to maximum depths of 350 {mu}m in human skin and 450 {mu}m in oral mucosa, with measured lateral resolution of 0.5{endash}1 {mu}m and axial resolution (section thickness) of 3{endash}5 {mu}m at the 1064-nm wavelength. This resolution is comparable with that of conventional microscopy of excised biopsies (histology). Normal and abnormal tissue morphology and dynamic processes were observed. {copyright} 1999 Optical Society of America
- DOE Contract Number:
- FG02-91ER61229
- OSTI ID:
- 341348
- Journal Information:
- Applied Optics, Journal Name: Applied Optics Journal Issue: 10 Vol. 38; ISSN APOPAI; ISSN 0003-6935
- Country of Publication:
- United States
- Language:
- English
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