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Process for selection of oxygen-tolerant algal mutants that produce H{sub 2}

Patent ·
OSTI ID:321237
;
A process for selection of oxygen-tolerant, H{sub 2}-producing algal mutant cells comprises: (a) growing algal cells photoautotrophically under fluorescent light to mid log phase; (b) inducing algal cells grown photoautotrophically under fluorescent light to mid log phase in step (a) anaerobically by (1) resuspending the cells in a buffer solution and making said suspension anaerobic with an inert gas and (2) incubating the suspension in the absence of light at ambient temperature; (c) treating the cells from step (b) with metronidazole, sodium azide, and added oxygen to controlled concentrations in the presence of white light; (d) washing off metronidazole and sodium azide to obtain final cell suspension; (e) plating said final cell suspension on a minimal medium and incubating in light at a temperature sufficient to enable colonies to appear; (f) counting the number of colonies to determine the percent of mutant survivors; and (g) testing survivors to identify oxygen-tolerant H{sub 2}-producing mutants. 5 figs.
Research Organization:
Midwest Research Institute
Sponsoring Organization:
USDOE, Washington, DC (United States)
DOE Contract Number:
AC36-83CH10093
Assignee:
Midwest Research Inst., Kansas City, MO (United States)
Patent Number(s):
US 5,871,952/A/
Application Number:
PAN: 8-835,897
OSTI ID:
321237
Country of Publication:
United States
Language:
English

References (7)

Induction, localization and metal content of hydrogenase in the green alga Chlamydomonas reinhardtii journal June 1994
Anionic modulation of the catalytic activity of hydrogenase from Chlamydomonas reinhardtii journal January 1982
A rapid procedure for selective enrichment of photosynthetic electron transport mutants. journal February 1977
Activation and De Novo Synthesis of Hydrogenase in Chlamydomonas journal December 1984
High-frequency nuclear transformation of Chlamydomonas reinhardtii. journal February 1990
[56] Chloroplasts: Formation of active oxygen and its scavenging book January 1984
Stabilization of Isolated Photosystem II Reaction Center Complex in the Dark and in the Light Using Polyethylene Glycol and an Oxygen-Scrubbing System journal February 1989

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Related Subjects

08 HYDROGEN
55 BIOLOGY AND MEDICINE
BASIC STUDIES
ALGAE
BATCH CULTURE
BIOSYNTHESIS
HYDROGEN PRODUCTION
MUTANTS