Establishment of quality assurance procedures for aquatic toxicity testing with the nematode Caenorhabditis elegans

Conference · Thu Dec 31 04:00:00 EST 1998

OSTI ID:320942

Freeman, M N; Marse, T J; Williams, P L ^[1]

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1. Univ. of Georgia, Athens, GA (United States). Environmental Health Science Program

In this study initial data were generated to develop laboratory control charts for aquatic toxicity testing using the nematode Caenorhabditis elegans. Tests were performed using two reference toxicants: CdCl{sub 2} and CuCl{sub 2}. All tests were performed for 24 h without a food source and of 48 h with a food source in a commonly used nematode aquatic medium. Each test was replicated 6 times with each replicate having 6 wells per concentration with 10 {+-} 1 worms per well. Probit analysis was used to estimate LC{sub 50} values for each test. The data were used to construct a mean ({bar x}) laboratory control chart for each reference toxicant. The coefficient of variation (CV) for three of the four reference toxicant tests was less than 20%, which demonstrates an excellent degree of reproducibility. These CV values are well within suggested standards for determination of organism sensitivity and overall test system credibility. A standardized procedure for performing 24 h and 48 h aquatic toxicity studies with C. elegans is proposed.

• OSTI ID: 320942
• Report Number(s): CONF-9704249--
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOASSAY
BIOLOGICAL INDICATORS
CADMIUM
COPPER
NEMATODES
QUALITY ASSURANCE
WATER POLLUTION