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Phylogenetic analysis of an anaerobic, trichlorobenzene-transforming microbial consortium

Journal Article · · Applied and Environmental Microbiology
OSTI ID:318831
;  [1]; ;  [2]
  1. Univ. Charite, Berlin (Germany). Inst. fuer Mikrobiologie und Hygiene
  2. Technische Univ. Berlin (Germany). Inst. fuer Technischen Umweltschutz

A culture-independent phylogenetic survey for an anaerobic trichlorobenzene-transforming microbial community was carried out. Small-subunit rRNA genes were PCR amplified from community DNA by using primers specific for Bacteria or Euryarchaeota and were subsequently cloned. Application of a new hybridization-based screening approach revealed 51 bacterial clone families, one of which was closely related to dechlorinating Dehalobacter species. Several clone sequences clustered to rDNA sequences obtained from a molecular study of an anaerobic aquifer contaminated with hydrocarbons and chlorinated solvents.

OSTI ID:
318831
Journal Information:
Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 1 Vol. 65; ISSN AEMIDF; ISSN 0099-2240
Country of Publication:
United States
Language:
English

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