Zymomonas mobilis oxidative stress transcriptomics

Dataset · Sun Aug 03 00:00:00 EDT 2025

DOI:https://doi.org/10.82250/3020578· OSTI ID:3020578

Askenasy, Isabel ^[1]; Myers, Kevin S. ^[2]

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1. DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA; Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA
2. DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA; Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, Wisconsin, USA

Zymomonas mobilis is an important bioenergy organism that has potential to produce biofuels, including ethanol, in high volumes. Here we examined the response of Zymomonas mobilis to various oxidative stresses using genome-scale transcriptomics data. We first examined the transcrpit abundance in WT aerobic growth compared to aerobic grown in paraquat, which forms superoxide. Under anaerobic growth conditions we compared WT Zymomonas mobilis with strains grown in media lacking iron as well as strains lacking iron that were treated with the iron chelator DIP before collection. Finally we examined transcript abundance in cells lacking ZMO_0422 (Rrf2 family transcription factor homolog) and ZMO_1411 (Fur homolog) grown under anaerobic conditions. Overall design: Transcriptomic analysis of WT, a deletion of ZMO_0422, and a deletion of ZMO_1411 in Zymomonas mobilis ZM4 under aerobic and anaerobic growth conditions along with various oxidative stresses: Paraquate addition, No Iron, and hydrogen peroxide addition.

• Research Organization: Great Lakes Bioenergy Research Center (GLBRC) (GLBRC)
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER)
• DOE Contract Number: SC0018409
• OSTI ID: 3020578
• Report Number(s): PRJNA1300971
• Country of Publication: United States
• Language: English

Related Subjects

aerobic
anaerobic
bioenergy
RNA-seq
stress response
transcriptomics
Zymomonas mobilis