Data for "Viral Delivery of Recombinases Activates Heritable Genetic Switches in Plants"
- Department of Genetics, Cell Biology and Development, College of Biological Sciences, University of Minnesota, Minneapolis, MN 55108, USA; Center for Precision Plant Genomics, University of Minnesota, Minneapolis, MN 55108, USA; Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55108, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Viral vectors provide an increasingly versatile platform for transformation-free reagent delivery to plants. RNA viral vectors can be used to induce gene silencing, overexpress proteins, or introduce gene editing reagents; however, they are often constrained by carrying capacity or restricted tropism in germline cells. Site-specific recombinases that catalyze precise genetic rearrangements are powerful tools for genome engineering that vary in size and, potentially, efficacy in plants. In this work, we show that viral vectors based on tobacco rattle virus (TRV) deliver and stably express four recombinases ranging in size from ∼0.6 to ∼1.5 kb and achieve simultaneous marker removal and reporter activation through targeted excision in transgenic Nicotiana benthamiana lines. TRV vectors with Cre, FLP, CinH, and Integrase13 efficiently mediated recombination in infected somatic tissue and led to heritable modifications at high frequency. An excision-activated Ruby reporter enabled simple and high-resolution tracing of infected cell lineages without the need for molecular genotyping. Together, our experiments broaden the scope of viral recombinase delivery and offer insights into infection dynamics that may be useful in developing future viral vectors.
- Research Organization:
- Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); University of Illinois Urbana-Champaign
- Sponsoring Organization:
- U.S. Department of Energy (DOE)
- DOE Contract Number:
- SC0018420
- OSTI ID:
- 3015302
- Country of Publication:
- United States
- Language:
- English
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Viral delivery of recombinases activates heritable genetic switches in plants
Journal Article
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Thu Feb 20 19:00:00 EST 2025
· Plant Physiology (Bethesda)
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OSTI ID:2522016