Microbial Co-Culture Control Proteomics (MC-DP1)

Dataset · Thu Jan 08 00:00:00 EST 2026

DOI:https://doi.org/10.25584/3013017· OSTI ID:3013017

Co-cultured S. elongatus PCC 7942 CscB/SPS and R. toruloides IFO0880 presented as an effective photosynthesis-driven biofuel production platform. The goal of this experiment was to understand the molecular mechanism of this co-culture system at redox post-translational modification level. The redox proteome of the co-cultured strains was compared to a mono-cultured S. elongatus in light or dark conditions. Samples were processed using a resin-assisted capture (RAC) workflow with TMT labeling to enrich and quantify modified cysteines at proteome level. The datasets were generated by a Q Exactive Plus Orbitrap Mass Spectrometer coupled with a Waters nanoAcquity UPLC, then searched by MSGF+ for downstream redox PTM analysis.

• Research Organization: PNNL (PNNL2)
• Sponsoring Organization: USDOE Office of Science (SC)
• Contributing Organization: Predictive Phenomics Initiative, Pacific Northwest National Laboratory
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 3013017
• Report Number(s): PNNL-SA-219351
• Country of Publication: United States
• Language: English